Iranian Researchers Create Method for Fast Cancer Cells Detection

Sunday, October 29, 2017 - 10:52

Researchers of Department of chemistry, University of Isfahan in cooperation with Cancer Research Centre of Lyon (CRCL), a research structure affiliated with the University Claude Bernard Lyon 1, have successfully presented an inexpensive and simple method for early detection of cancer cells.

According to an ISCA report, the researchers introduced a sensitive and selective signal-on method for aptamer based spectrofluorometric detection of cancer cells.

Cancer is a major cause of mortality worldwide and its early diagnosis significantly increases patient survival rates1. Most biochemical analysis techniques employed to detect cancer cells are based on the use of specific ligands for protein recognition.

Several labeling techniques, such as fluorescent, chemiluminescent, radioactive, and electrochemical, labeling have been developed for cancer cell detection at the molecular level. However, applications for such methods remain limited owing to their elevated cost and complexity.

In this regard, Assistant Professor of chemistry department of University of Isfahan, Masoud A. Mehrgardi stated that the project has been carried out in laboratory scale and hoped that through its development, it would be used in early cancer cells diagnosis.

He explained that the study introduced a sensitive and selective signal-on method for aptamer based spectrofluorometric detection of cancer cells. Aptamer-based spectrofluorometric assays offer one of the most sensitive protocols for the detection of cancer cells.

The efficiency of spectrofluorometric protocols can be further improved by the use of nanostructures, as evidenced by the successful application of aptamer-conjugated fluorescence silica nanoparticles, CdSe/ZnS core/shell quantum dots and carbon nanodots, for the sensitive monitoring of cancer cells.

Mouse breast tumor cells (4T1), human breast tumor cells (MCF7), and human cervical cancer cells (HeLa), all of which overexpress nucleolin on their surface, were incubated in the presence of control human foreskin fibroblast cells (HFFF-PI6) and CDs-AS1411 aptamer probes to investigate the sensitivity and selectivity of our signal-on spectrofluorometric assay for the targeted detection of cancer cells.

The investigation was done by Hasan Motaghi, PhD. student of chemistry department of Isfahan University, Masoud Ayatollahi Mehrgardi, assistant professor, and Philippe Bouvet, Molecular Biology professor of CRCL.


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