Shushtar IAU Researcher Reproduces Rare Plants with Tissue Culture Technique

Tuesday, December 19, 2017 - 15:46

Researchers of Islamic Azad University, Shushtar branch, have succeeded to reproduce and expand rare plants through tissue culture technique.

According to an ISCA report, Mohammad Deli, head of Royan Kesht Amordad Company, the core technology located in Shushtar IAU, explained that plant tissue culture is defined as culturing plant seeds, organs, explants, tissues, cells, or protoplasts on a chemically defined synthetic nutrient media under sterile and controlled conditions of light, temperature, and humidity.

Plant tissue cultures can be initiated from almost any part of a plant. The physiological state of the plant does have an influence on its response to attempts to initiate tissue culture. The parent plant must be healthy and free from obvious signs of disease or decay. The source, termed explant, may be dictated by the reason for carrying out the tissue culture. Younger tissue contains a higher proportion of actively dividing cells and is more responsive to a callus initiation program. The plants themselves must be actively growing, and not about to enter a period of dormancy.

He added that the plant is growing through stimulating its hormones in Murashige and Skoog medium (or MSO or MS0 (MS-zero)) in the laboratories. It must be noted that there is no exchange of gases with the external environment, and the required nutrition is in plant storage containers.


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