Tehran IAU Medical Branch Could Isolate the Pigments from the Psychrotrophic Bacteria

Monday, January 1, 2018 - 10:30

Researchers from Islamic Azad University, Neyshabur and Shahrood branch, have carried out a research about Isolation and assessment of the pigments from the psychrotrophic bacteria with the aim of application in the food industries.

According to an ISCA report, nowadays, the use of the organic pigments produced by the microorganisms according ‎to their nature and safety is increasing. These pigments are various in their nature and have different ‎properties.

The research aimed at extracting the new bacterial pigments that are produced ‎in the cold environments. Researchers collected and ‎cultivated ten soil samples from different areas of Torghabeh city. After 10 days, 14 pigmented bacterial colonies were selected then the isolates were ‎identified using the biochemical tests and polymerase chain reaction (PCR).

Using the methanol and ‎glass powder, bacterial pigments were extracted and in order to purification TLC was performed. ‎Pigments of two strains, A8 and A13, which had a suitable purity, were used for cell toxicity using ‎three cell lines HepG2, human embryonic fibroblasts, and gastric cancer. ‎

results show that among 14 pigmented bacterial strains, the strain A8 that was Aeromonas hydrophyla, could ‎prevent the growth of the cells on different cancerous cell line. Also, the pigment that was named A13, ‎had no cytotoxic effects on all the used cell types and can be used as a natural coloring agent in the ‎food industry. ‎

Based on the biological properties of the extracted bacterial ‎pigments, they can be used in different areas such as medicine and technology.‎

The reseach was carried out by Hadise Pazoki, MSc Microbiology, Department of Biology of Neyshabur IAU, and Parastoo Pourali, Assistant Professor‎ Department of basic science, Faculty of medical science of Shahrood IAU.

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