Shabestar IAU Faculty Member Presents a New Protocol of Type 2 Diabetes Induction

Monday, January 15, 2018 - 11:55

A faculty member from Islamic Azad University, Shabestar branch, has introduced a new protocol of Type 2 Diabetes induction and hydrocortisone effects on beta-cells signaling in mice which was published in Current Chemical Biology Journal.

According to an ISCA report, there are complex methods to induce of type 2 diabetes such as multiple low-doses streptozotocin (MLDS) with high fat diet administration and others which induces modest DNA damage, leads to incomplete destruction of β cells for insulin-resistant diabetes in mice.

Dr. Tohid Vahdatpour investigated an innovative and non-complex protocol to induce type 2 diabetes by incremental multiple low-doses streptozotocin (IMLDS). The result of this research was published in Current Chemical Biology Journal.

Based on the research method, IMLDS designed protocol was conducted in 5 consecutive days by STZ injections of 20, 40, 80 and 160 mg/kg BW/day and stress induced by HC injections of 5, 10 and 20 mg/kg BW/day in mice. The glucose, insulin, glucagon-like peptide 1 (GLP-1), RAC-beta serine/threonine-protein kinase (AKT-2) and dipeptidyl peptidase IV (DPP-4) of blood were assessed by ELISA.

The result shows that the glucose, GLP-1, AKT-2 levels, and food and water intake increased (p<0.01) while serum DPP-4 activity decreased (p<0.01) after administration of STZ. Daily injections of HC were raised blood glucose, GLP-1and AKT-2 and reduced serum insulin and DPP-4 (p<0.05).

Exclusive administration of STZ base on new protocol increased the serum insulin, whereas HC administration with STZ decreased the insulin (p<0.05). The HC administration reduced GLP-1 in the absence of STZ, but HC enhanced GLP-1 after STZ injections (p<0.05).

This new protocol may provide a practical and unique alternative chemical protocol for induction of insulin resistance diabetes and stress induced by HC could intensify type 2 diabetes induced by this new method in the mice.

The research was carried out by Tohid Vahdatpour and faculty of Veterinary Medicine, Tabriz IAU, Hamid Mahmoudpour.


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