Shabestar IAU Faculty Member Presents a New Protocol of Type 2 Diabetes Induction

Monday, January 15, 2018 - 11:55

A faculty member from Islamic Azad University, Shabestar branch, has introduced a new protocol of Type 2 Diabetes induction and hydrocortisone effects on beta-cells signaling in mice which was published in Current Chemical Biology Journal.

According to an ISCA report, there are complex methods to induce of type 2 diabetes such as multiple low-doses streptozotocin (MLDS) with high fat diet administration and others which induces modest DNA damage, leads to incomplete destruction of β cells for insulin-resistant diabetes in mice.

Dr. Tohid Vahdatpour investigated an innovative and non-complex protocol to induce type 2 diabetes by incremental multiple low-doses streptozotocin (IMLDS). The result of this research was published in Current Chemical Biology Journal.

Based on the research method, IMLDS designed protocol was conducted in 5 consecutive days by STZ injections of 20, 40, 80 and 160 mg/kg BW/day and stress induced by HC injections of 5, 10 and 20 mg/kg BW/day in mice. The glucose, insulin, glucagon-like peptide 1 (GLP-1), RAC-beta serine/threonine-protein kinase (AKT-2) and dipeptidyl peptidase IV (DPP-4) of blood were assessed by ELISA.

The result shows that the glucose, GLP-1, AKT-2 levels, and food and water intake increased (p<0.01) while serum DPP-4 activity decreased (p<0.01) after administration of STZ. Daily injections of HC were raised blood glucose, GLP-1and AKT-2 and reduced serum insulin and DPP-4 (p<0.05).

Exclusive administration of STZ base on new protocol increased the serum insulin, whereas HC administration with STZ decreased the insulin (p<0.05). The HC administration reduced GLP-1 in the absence of STZ, but HC enhanced GLP-1 after STZ injections (p<0.05).

This new protocol may provide a practical and unique alternative chemical protocol for induction of insulin resistance diabetes and stress induced by HC could intensify type 2 diabetes induced by this new method in the mice.

The research was carried out by Tohid Vahdatpour and faculty of Veterinary Medicine, Tabriz IAU, Hamid Mahmoudpour.

Opinions


Popular News

Latest News