S & R IAU Researcher Detects Salmonella Typhimurium and Salmonella Enteritidis in Raw Egg

Sunday, April 15, 2018 - 11:53

A researcher from Islamic Azad University, Science and Research branch, has succeeded to separate and recognize Salmonella Typhimurium and Salmonella Enteritidis in raw egg through designing of real-time PCR method and HRM analysis.

According to an ISCA report, Sabereh Fahimi stated that Salmonella is one of the most important causes of food poisoning and recognition of Salmonella food typically culture, is a time-consuming method and is not accurate enough.

As she said, this recent research has presented a fast and separation on this bacteria in eggs. Based on the research, sample eggs of without Salmonella inoculated with Salmonella Typhimurium, Salmonella Enteritidis and a mixture of both bacteria and finally real-time PCR method designed with using a pair of special primer for recognition of mention bacteria and HRM analysis was used for separation of genealogy.

Real-Time PCR method and HRM could identify and separate bacteria Salmonella Typhimurium, Salmonella Enteritidis and a mixture of both in the eggs content with minimum identifiable 200 Salmonella bacteria with common deigned primer.

The research has been carried out by Sabereh Fahimi and led by Hamed Ahari and Nariman Sheikhi in Science and Research IAU and published in Scientific Information Database (SID), one of the best comprehensive up-to-date data banks in Iran.

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