Iranian Researchers Detect Alzheimer's Disease Using a Biosensor

Tuesday, May 1, 2018 - 10:46

Researchers from Shahid Sadoughi University of Medical Sciences and Health Services in Yazd and University of Tehran have succeeded to make a biosensor which provides an opportunity to detect Alzheimer's disease.

According to an ISCA report, Alzheimer's disease (AD) is the most common form of dementia, which its early detection through using reliable molecular biomarkers, is believed to be the best approach to controlling and even curing it.

Herein, we develop an ultrasensitive electrochemical nanobiosensor to quantify serum miR-137 as a validated biomarker of AD. Electrochemically-reduced graphene oxide (ERGO) and gold nanowires (AuNWs) are used to modify the surface of a screen-printed carbon electrode (SPCE) with the application of an intercalated label, doxorubicin (Dox), explained, Dr. Mostafa Azimzadeh, faculty member of Shahid Sadoughi University of Medical Sciences and Health Services in Yazd.

Based on the research, the fabrication steps are analyzed via field emission scanning electron microscopy (FE-SEM) and energy dispersive spectroscopy (EDS), as well as two reliable electrochemical methods, cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS).

The results show that the linear range of the nanobiosensor is from 5.0 to 750.0 fM and its limit of detection is 1.7 fM. In addition, the nanobiosensor shows a great performance in specificity experiments and is able to discriminate between target oligos versus non-specific oligos (one-base mismatch target; three-base mismatch target, non-specific miR-21 and miR-155) very well. The evaluation of the sensing mechanism of the developed nanobiosensor in actual human serum reveals its potential clinical applications for the early detection of AD in the future.

The research was done by Dr. Mostafa Azimzadeh, Dr. Navid Nasirizadeh, faculty member of Yazd Islamic Azad University, Dr. Mahdi Rahaie, faculty member of University of Tehran and Hossein Naderi-Manesh, faculty member of Tarbiat Modares University and its result is available in RSC Advances.


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