Iranian Researchers Discover a Novel Cell to Treat Parkinson Disease

Tuesday, June 26, 2018 - 16:06

Iranian researchers have discovered novel cell surface markers for purification of embryonic dopamine progenitors for transplantation in Parkinson’s disease animal models.

According to an ISCA report, Parkinson's disease is due to the loss of brain cells that produce dopamine. Despite the progress in safety and efficacy of cell replacement therapy with pluripotent stem cells (PSCs), the presence of residual undifferentiated stem cells or proliferating neural progenitor cells (NPCs) with rostral identity remains a major challenge.

In this regard, researchers report the generation of a LIM homeobox transcription factor 1 alpha (LMX1A) knock-in GFP reporter human embryonic stem cell (hESC) line that marks the early dopaminergic progenitors during neural differentiation to find reliable membrane protein markers for isolation of midbrain dopaminergic neurons.

Purified GFP positive cells in vitro exhibited expression of mRNA and proteins that characterized and matched the midbrain dopaminergic identity. Further quantitative proteomics analysis of enriched LMX1A+ cells identified several membrane-associated proteins including polysialylated embryonic form of neural cell adhesion molecule (PSA-NCAM) and contactin 2 (CNTN2), enabling prospective isolation of LMX1A+ progenitor cells.

Transplantation of hPSC-derived purified CNTN2+ progenitors enhanced dopamine release from transplanted cells in the host brain and alleviated Parkinson’s disease-related phenotypes in animal models. The study establishes an efficient approach for purification of large numbers of hPSC-derived dopaminergic progenitors for therapeutic applications.

The research has been carried out by Ali Fathi, Mehdi Mirzaei, Banafsheh Dolatyar, Mehdi Sharifitabar, Mahnaz Bayat, Ebrahim Shahbazi, Jaesuk Lee, Mohammad Javan, Su-Chun Zhang, Vivek Gupta, Bonghee Lee, Paul A. Haynes, Hossein Baharvand, and Ghasem Hosseini Salekdeh.

The result of the study published on Molecular & Cellular Proteomics journals.


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