IAU Researchers Record 11 Genes in NCBI

Tuesday, September 11, 2018 - 13:09

Researchers of Islamic Azad University, Khorramabad branch, have successfully recorded 11 genes in National Center for Biotechnology Information.

According to an ISCA report, a team of researchers from Khorramabad IAU, Department of Agronomy and Plant Breeding, could record 11 genes in NCBI by carrying out a research project, “Identification and bioinformatics analysis of defensin antimicrobial genes from six lentil genotypes.”

The team included Reza Mir Derikvand, assistant professor in Agronomy and Plant Breeding Department of Khorramabad IAU, Seyyed Mohsen Sohrabi, researcher of Young Researchers and Elite Club and Kamran Samiei, assistant professor in Department of Agronomy and Plant Breeding, Kangavar IAU.

Based on the research, Plant defensins are a large family of cysteine-rich antimicrobial peptides. These peptides are small molecules with molecular mass between 5 and 7kD and eight conserved cysteines that are involved in disulfide bond formation.

In the study, some members of defensin gene family were isolated and characterized from lentil genotypes. All coding sequences of the defensin genes of dicotyledon plants were retrieved from GenBank.

The retrieved coding sequences were aligned against lentil EST library and the resulted sequences were pooled and assembled. The resulted contigs and singletons were aligned against GenBank nr database using BLASTn tool. The contigs and singletons with the full open reading frame were used to primer design.

In the next step, DNA extraction was performed from Gachsaran, Mahali, Filip2003-2L, Filip2003-9L, Filip2005-2L and Filip2006-10L genotypes. Finally, by using PCR, genomic sequence of defensins were amplified, cloned into pTZ57R/T plasmid and sequenced.

The results showed complete identity among the isolated defensins from all genotypes. The identified genes also contain conserved introns with variable length that comprised of regulatory elements for response to different factors and conditions. For first time in this study, genomic sequences of three members of defensin gene family were isolated from lentil genotypes and structures and features of them were determined.

1 Linear mRNA 520 bp M 325793 Locus

PLN 30-AUG-2018

2 Linear mRNA 534 bp M 325792 Locus

PLN 30-AUG-2018

3 Linear mRNA 516 bp M 325791 Locus

PLN 30-AUG-2018

4 Linear mRNA 702 bp M 325790 Locus

PLN 30-AUG-2018

5 Linear mRNA 492 bp M 325789 Locus

PLN 30-AUG-2018

6 Linear mRNA 343 bp M 325788 Locus

PLN 30-AUG-2018

7 Linear mRNA 520 bp M 325787 Locus

PLN 30-AUG-2018

8 Linear mRNA 443 bp M 325786 Locus

PLN 30-AUG-2018

9 Linear mRNA 440 bp M 325785 Locus

PLN 30-AUG-2018

10 Linear mRNA 501 bp M 325784 Locus

PLN 30-AUG-2018

11 Linear mRNA 488 bp M 325783 Locus

PLN 30-AUG-2018

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